Slides for university students are usually prepared in the histology lab. Students in medical sciences are often trained to know what a tissue is when placed on a slide. The essence is for them to be able to identify anomalies or deviations from the standard ones. However, before they can start taking any of the required lectures, the lab technologists must take the tissues through some histology techniques to prepare microscope slides.
There are different kinds of microscopes the students can use in the lab. The ones to be used should depend on what they will be asked to identify as well as the conditions in the laboratory. They include the electron, light, phase contrast, fluoresce, confocal microscopes.
The parts of a light microscope include the presence of lamp, iris, diaphragm, tube and lenses, eyepiece, adjustment knobs, and objective lens. There are usually three types of adjustment knobs, and they include condenser height, fine and coarse knobs. Their major function is to control the clarity of what is being viewed.
The tissues that will be placed under the microscope must be processed before they can be used. There are different techniques to do this and they include fixation, dehydration, clearing, waxing, embedding, and staining. The use of chemicals applies in these steps and they have to be in the required amount if the tissue is going to be well prepared or last long.
Fixation is the first step to take after the death of a cell. It is done to prevent decay since tissues start breaking down immediately after death. The dead tissue is soaked in fixatives such as buffered formalin, Bouin's fluid, and salt. Other ways to prevent decay of tissues after death are refrigeration and heating. When applying fixatives, the ratio should be 3 volumes of fixatives to 1 volume of tissue. This volume must be maintained to make sure the whole tissue is covered.
Fixation usually introduces water that must be removed through the process called dehydration. Since alcohol and water are miscible, alcohol can be used in the process. The concentration of alcohol used should vary from low to high and in an ascending order. It is good to do it with 50%, 50%, 75%, 75%, 98%, and 98% alcohol concentrations one after the other. This is necessary to remove all bubbles and to prevent the possibility of shrinking.
The alcohol introduced into the tissue must not be left for long. The process involved in getting rid of it is known as clearing. It is done with clearing agents such as xylene, benzene, chloroform, and toluene. After this, wax is impregnated into the tissues so as to remove xylene. In addition to xylene removal, waxing makes it easy to cut the tissues and the cuts are also strong.
The slides that will be viewed should not contain wax. Thus, it should be removed by the process of rehydration. Rehydration is done with alcohol in descending grades; that is from absolute to 50 percent. Water can be reintroduced since the tissue must be brought back to water. Staining is the final process in the technique and the stains used depends on the tissue and what should be identified. They include Periodic Acid Schiff, Sudan black, Van Gieson and Osmium tetroxide.
There are different kinds of microscopes the students can use in the lab. The ones to be used should depend on what they will be asked to identify as well as the conditions in the laboratory. They include the electron, light, phase contrast, fluoresce, confocal microscopes.
The parts of a light microscope include the presence of lamp, iris, diaphragm, tube and lenses, eyepiece, adjustment knobs, and objective lens. There are usually three types of adjustment knobs, and they include condenser height, fine and coarse knobs. Their major function is to control the clarity of what is being viewed.
The tissues that will be placed under the microscope must be processed before they can be used. There are different techniques to do this and they include fixation, dehydration, clearing, waxing, embedding, and staining. The use of chemicals applies in these steps and they have to be in the required amount if the tissue is going to be well prepared or last long.
Fixation is the first step to take after the death of a cell. It is done to prevent decay since tissues start breaking down immediately after death. The dead tissue is soaked in fixatives such as buffered formalin, Bouin's fluid, and salt. Other ways to prevent decay of tissues after death are refrigeration and heating. When applying fixatives, the ratio should be 3 volumes of fixatives to 1 volume of tissue. This volume must be maintained to make sure the whole tissue is covered.
Fixation usually introduces water that must be removed through the process called dehydration. Since alcohol and water are miscible, alcohol can be used in the process. The concentration of alcohol used should vary from low to high and in an ascending order. It is good to do it with 50%, 50%, 75%, 75%, 98%, and 98% alcohol concentrations one after the other. This is necessary to remove all bubbles and to prevent the possibility of shrinking.
The alcohol introduced into the tissue must not be left for long. The process involved in getting rid of it is known as clearing. It is done with clearing agents such as xylene, benzene, chloroform, and toluene. After this, wax is impregnated into the tissues so as to remove xylene. In addition to xylene removal, waxing makes it easy to cut the tissues and the cuts are also strong.
The slides that will be viewed should not contain wax. Thus, it should be removed by the process of rehydration. Rehydration is done with alcohol in descending grades; that is from absolute to 50 percent. Water can be reintroduced since the tissue must be brought back to water. Staining is the final process in the technique and the stains used depends on the tissue and what should be identified. They include Periodic Acid Schiff, Sudan black, Van Gieson and Osmium tetroxide.
About the Author:
When you are searching for information about microscope slides, come to our web pages today. More details are available at http://www.greengeological.com/shop/category/35-microscope-slides now.
Posts
No comments:
Post a Comment